1F0M
MONOMERIC STRUCTURE OF THE HUMAN EPHB2 SAM (STERILE ALPHA MOTIF) DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-09-08 |
| Detector | OTHER |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 54.837, 54.837, 65.545 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.26400 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.097 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 50.000 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.048 | 0.302 * |
| Number of reflections | 4963 | |
| <I/σ(I)> | 31 | |
| Completeness [%] | 92.0 | 91.1 |
| Redundancy | 8.4 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% peg, 70 mM lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 25K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | |
| 3 | 1 | drop | 87.5 (mM) | ||
| 4 | 1 | drop | PEG6000 | 15 (%) | |
| 5 | 1 | reservoir | HEPES | 100 (mM) | |
| 6 | 1 | reservoir | 175 (mM) | ||
| 7 | 1 | reservoir | PEG6000 | 30 (%) |
