1F0J
CATALYTIC DOMAIN OF HUMAN PHOSPHODIESTERASE 4B2B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-07-26 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 104.540, 159.580, 109.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.000 - 1.770 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.22300 |
RMSD bond length | 0.005 |
RMSD bond angle | 19.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.060 | 0.452 |
Number of reflections | 88528 | |
<I/σ(I)> | 30.3 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 282 | PEG, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 282K |
1 | Vapor diffusion * | 7 | 282 | PEG, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 282K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | PEG3000 | precipitant |