1F0B
CRYSTAL STRUCTURE OF THE GREEN FLUORESCENT PROTEIN (GFP) VARIANT YFP-H148Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-04 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.661, 62.646, 66.183 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.500 - 2.100 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.060 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.500 | 2.150 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.083 | 0.320 |
| Total number of observations | 29297 * | |
| Number of reflections | 11419 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 82.1 | 81.5 |
| Redundancy | 2.56 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 * | 4 * | PEG 1550, sodium acetate, magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | reservoir | PEG1550 | 22 (%(w/v)) | |
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 5 | 1 | reservoir | 90 (mM) |
