1F09

CRYSTAL STRUCTURE OF THE GREEN FLUORESCENT PROTEIN (GFP) VARIANT YFP-H148Q WITH TWO BOUND IODIDES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-08-30
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	51.197, 62.818, 68.667
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.100 - 2.140
R-factor	0.204
Rwork	0.204
RMSD bond length	0.016
RMSD bond angle	2.830
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.100	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.090	0.519
Total number of observations	35882 *
Number of reflections	10849
<I/σ(I)>	6.7
Completeness [%]	85.6	79.6
Redundancy	3.31	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9 *	4 *	PEG 1550, sodium acetate, magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris	20 (mM)
3	1	reservoir	PEG1550	22 (%(w/v))
4	1	reservoir	sodium acetate	100 (mM)
5	1	reservoir		90 (mM)