1F08

CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-26
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	42.170, 84.897, 124.276
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.900
R-factor	0.241 *
Rwork	0.241
R-free	0.27100
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	22.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.087	0.418
Total number of observations	332339 *
Number of reflections	35804	3500 *
<I/σ(I)>	11.9	4.35
Completeness [%]	99.2	96.4
Redundancy	9.28	6.05

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	100 mM MES, pH 6.5, NaBr or NaCl, 8 mg/mL protein, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	100 mM MES, pH 6.5, NaBr or NaCl, 8 mg/mL protein, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)