1F06
THREE DIMENSIONAL STRUCTURE OF THE TERNARY COMPLEX OF CORYNEBACTERIUM GLUTAMICUM DIAMINOPIMELATE DEHYDROGENASE NADPH-L-2-AMINO-6-METHYLENE-PIMELATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1999-07-28 |
Detector | XENTRONICS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.547, 65.591, 84.281 |
Unit cell angles | 90.00, 106.52, 90.00 |
Refinement procedure
Resolution | 48.600 - 2.100 |
R-factor | 0.178 * |
Rwork | 0.179 |
R-free | 0.20900 |
RMSD bond length | 0.007 |
RMSD bond angle | 26.400 * |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.600 | 2.250 |
High resolution limit [Å] | 2.100 | 2.110 |
Rmerge | 0.076 | 0.224 |
Number of reflections | 39441 * | |
<I/σ(I)> | 8.615 | |
Completeness [%] | 86.9 | 67 * |
Redundancy | 2.82 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG 8000, magnesium acetate, cacodylate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 14 (%) | |
2 | 1 | reservoir | magnesium acetate | 200 (mM) | |
3 | 1 | reservoir | sodium acetate | 100 (mM) | |
4 | 1 | drop | protein | 24 (mg/ml) | |
5 | 1 | drop | HEPES | 20 (mM) | |
6 | 1 | drop | NADPH | 5 (mM) | |
7 | 1 | drop | L-2-amino-6-methylene-pimelate | 20 (mM) |