1F00
CRYSTAL STRUCTURE OF C-TERMINAL 282-RESIDUE FRAGMENT OF ENTEROPATHOGENIC E. COLI INTIMIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-05 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.160, 47.100, 71.660 |
Unit cell angles | 90.00, 94.20, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.25500 |
RMSD bond length | 0.051 |
RMSD bond angle | 1.419 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.097 | 0.261 |
Number of reflections | 24955 | |
<I/σ(I)> | 15.1 | |
Completeness [%] | 90.7 | 51.5 |
Redundancy | 3.73 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 1.8 M potassium phosphate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 1.8 (M) | or 2.5M ammonium sulfate |