1F00
CRYSTAL STRUCTURE OF C-TERMINAL 282-RESIDUE FRAGMENT OF ENTEROPATHOGENIC E. COLI INTIMIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-05 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.160, 47.100, 71.660 |
| Unit cell angles | 90.00, 94.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25500 |
| RMSD bond length | 0.051 |
| RMSD bond angle | 1.419 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.097 | 0.261 |
| Number of reflections | 24955 | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 90.7 | 51.5 |
| Redundancy | 3.73 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 1.8 M potassium phosphate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 1.8 (M) | or 2.5M ammonium sulfate |
