1EZK
Crystal structure of recombinant tryparedoxin I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-13 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.104, 39.730, 99.173 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.198 * |
Rwork | 0.198 |
R-free | 0.28300 |
RMSD bond length | 0.026 |
RMSD bond angle | 0.044 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.500 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.069 * | 0.128 |
Number of reflections | 11980 | |
<I/σ(I)> | 5.2 | |
Completeness [%] | 96.5 | 81.1 |
Redundancy | 4.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.7 * | 294 * | Kalisz, H.M., (1998) Acta Crystallogr., Sect.D, 55, 696. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 13.5 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 33 (%(w/w)) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.7 |
4 | 1 | reservoir | sodium acetate | 50 (mM) |