1EZI
Structure of a sialic acid activating synthetase, CMP acylneuraminate synthetase in the presence and absence of CDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-04-22 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.980, 59.520, 157.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.000 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.26700 * |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.230 |
High resolution limit [Å] | 2.000 * | 2.100 |
Rmerge | 0.073 * | 0.220 |
Number of reflections | 25606 * | |
<I/σ(I)> | 18.6 | |
Completeness [%] | 99.0 * | 83.4 |
Redundancy | 8.0 * | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 291 | 28% PEG 2000, 100 mM Tris-HCl, 1 % Ethanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5.0 (mg/ml) | |
10 | 1 | reservoir | CDP | 10 (mM) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | EDTA | 0.1 (mM) | |
6 | 1 | reservoir | PEG2000 | 16 (%) | |
7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
8 | 1 | reservoir | 10 (mM) | ||
9 | 1 | reservoir | dithiothreitol | 1 (mM) |