1EZG
CRYSTAL STRUCTURE OF ANTIFREEZE PROTEIN FROM THE BEETLE, TENEBRIO MOLITOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-10 |
Detector | MARRESEARCH |
Spacegroup name | P 65 |
Unit cell lengths | 73.832, 73.832, 53.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 5.000 - 1.400 |
R-factor | 0.16 |
Rwork | 0.161 |
R-free | 0.19960 |
RMSD bond length | 0.011 |
RMSD bond angle | 2.154 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.056 | 0.208 |
Number of reflections | 32557 | |
<I/σ(I)> | 10.1 | |
Completeness [%] | 96.5 | 95.6 |
Redundancy | 4.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 * | 295 | ammonium sulfate, sodium citra, cobalt chloridee, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | reservoir | 1.5 (M) | ||
3 | 1 | reservoir | glycine-NaOH | 100 (mM) |