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1EZ8

STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RUH3R
Temperature [K]297
Detector technologyIMAGE PLATE
DetectorRIGAKU RAXIS IV
Spacegroup nameP 41
Unit cell lengths48.158, 48.158, 63.708
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution8.000 - 1.850
R-factor0.1618

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R-free0.26500
Structure solution methodAB INITIO
RMSD bond length0.006
RMSD bond angle0.023
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSHELX
Refinement softwareSHELXL-97
Data quality characteristics
 Overall
Low resolution limit [Å]8.000

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High resolution limit [Å]1.850

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Completeness [%]92.9

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Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7277MPD, Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirMPD40-60 (%(v/v))
21reservoirsodium phosphate25 (mM)

222415

PDB entries from 2024-07-10

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