1EZ3
CRYSTAL STRUCTURE OF THE NEURONAL T-SNARE SYNTAXIN-1A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-02-27 |
Detector | OTHER |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.410, 99.560, 54.960 |
Unit cell angles | 90.00, 97.46, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.233 |
Rwork | 0.231 |
R-free | 0.28640 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.748 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.046 | 0.423 |
Total number of observations | 155471 * | |
Number of reflections | 48323 | |
<I/σ(I)> | 37.9 | |
Completeness [%] | 97.4 | 78.2 |
Redundancy | 4.53 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 23 * | PEG 1500, sodium acetate, DTT, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | drop | dithiothreitol | 10 (mM) | |
3 | 1 | reservoir | PEG1500 | 16 (%) | |
4 | 1 | reservoir | sodium acetate | 20 (mM) | pH5.0 |
5 | 1 | reservoir | dithiothreitol | 10 (mM) |