1EYY
CRYSTAL STRUCTURE OF THE NADP+ DEPENDENT ALDEHYDE DEHYDROGENASE FROM VIBRIO HARVEYI.
Replaces: 1CBZExperimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 1997-07-24 |
Detector | BRANDEIS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.550, 131.360, 92.800 |
Unit cell angles | 90.00, 92.44, 90.00 |
Refinement procedure
Resolution | 25.770 - 2.500 |
Rwork | 0.206 |
R-free | 0.23900 |
Structure solution method | MIR AND MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 22.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.200 | 2.570 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.056 | 0.118 |
Total number of observations | 218666 * | |
Number of reflections | 130457 | |
<I/σ(I)> | 14.7 | |
Completeness [%] | 98.1 | 95.3 |
Redundancy | 1.67 | 1.622 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | Croteau, N., (1996) Protein Sci., 5, 2130. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 18-30 (%(w/v)) | |
2 | 1 | reservoir | sodium acetate | 200 (mM) | |
3 | 1 | reservoir | sodium cacodylate | 100 (mM) |