1EYX
CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-31 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 187.268, 187.268, 59.109 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 999.000 - 2.250 |
| R-factor | 0.192 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lia |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.012 |
| Data reduction software | XDS (V. 1998) |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.550 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.101 | 0.039 |
| Total number of observations | 123143 * | |
| Number of reflections | 38751 | |
| <I/σ(I)> | 7.3 | 2.8 |
| Completeness [%] | 98.7 | 89.4 |
| Redundancy | 3.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 * | 200 MM HEPES, 500 MM SODIUM CHLORIDE, 50 MM POTASIUM CHLORIDE, 15 MM SODIUM AZIDE, 16% AMMONIUM SULPHATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12-15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 16-18 (%sat) | |
| 3 | 1 | reservoir | HEPES | 200 (mM) | |
| 4 | 1 | reservoir | sodium chloride | 500 (mM) | |
| 5 | 1 | reservoir | sodium azide | 15 (mM) |
