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1EYX

CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE BM14
Synchrotron siteESRF
BeamlineBM14
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date1998-08-31
DetectorMARRESEARCH
Spacegroup nameH 3
Unit cell lengths187.268, 187.268, 59.109
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution999.000 - 2.250
R-factor0.192
R-free0.27900
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1lia
RMSD bond length0.004
RMSD bond angle0.012
Data reduction softwareXDS (V. 1998)
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareSHELXL-97
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]42.5502.280
High resolution limit [Å]2.1602.160
Rmerge0.1010.039
Total number of observations123143

*

Number of reflections38751
<I/σ(I)>7.32.8
Completeness [%]98.789.4
Redundancy3.12
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7294

*

200 MM HEPES, 500 MM SODIUM CHLORIDE, 50 MM POTASIUM CHLORIDE, 15 MM SODIUM AZIDE, 16% AMMONIUM SULPHATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein12-15 (mg/ml)
21reservoirammonium sulfate16-18 (%sat)
31reservoirHEPES200 (mM)
41reservoirsodium chloride500 (mM)
51reservoirsodium azide15 (mM)

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