1EYU
HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-12 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.797, 84.336, 46.155 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.780 |
R-factor | 0.24 |
Rwork | 0.208 |
R-free | 0.24300 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.810 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.045 | 0.198 |
Total number of observations | 130055 * | |
Number of reflections | 35254 | |
<I/σ(I)> | 3621.7 | |
Completeness [%] | 98.0 | 96.5 |
Redundancy | 3.7 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 16 * | Balendiran, K., (1994) Proteins: Struct. Funct. Genet., 19, 77. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | sodium acetate | ||
2 | 1 | 1 | CaCl2 | ||
3 | 1 | 1 | PEG 4000 | ||
4 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9.6 (mg/ml) | |
2 | 1 | drop | duplex DNA | 1.6 (mg/ml) | |
3 | 1 | drop | 25 (mM) | ||
4 | 1 | drop | PEG4000 | 18-25 (%) | mother liquor |
5 | 1 | drop | EDTA | 0.3 (mM) | mother liquor |
6 | 1 | drop | sodium acetate | 50 (mM) | mother liquor |
7 | 1 | reservoir | mother liquor | 31-45 (%) |