1EYT
CRYSTAL STRUCTURE OF HIGH-POTENTIAL IRON-SULFUR PROTEIN FROM THERMOCHROMATIUM TEPIDUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1999-11-17 |
| Detector | WEISSENBERG |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.118, 59.592, 23.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.213 |
| Rwork | 0.212 |
| R-free | 0.23800 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 26.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.067 | 0.219 |
| Total number of observations | 50767 * | |
| Number of reflections | 10306 * | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 86.7 | 67.6 |
| Redundancy | 4.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 3.5 | 297 * | Nogi, T., (2000) Acta Crystallogr., Sect.D, 56, 656. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 0.7 (M) | |
| 3 | 1 | drop | sodium citrate | 0.05 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) |
