1EYS
CRYSTAL STRUCTURE OF PHOTOSYNTHETIC REACTION CENTER FROM A THERMOPHILIC BACTERIUM, THERMOCHROMATIUM TEPIDUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-25 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 133.299, 196.596, 84.163 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.234 |
| Rwork | 0.231 |
| R-free | 0.28700 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.093 | 0.362 |
| Total number of observations | 508729 * | |
| Number of reflections | 110923 * | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 92.6 | 78.6 |
| Redundancy | 4.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 277 | Kobayashi, M., (1993) Bull. Chem. Soc. Jpn., 66, 3834. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | drop | 0.36 (M) | ||
| 3 | 1 | reservoir | phosphate | 15 (mM) | |
| 4 | 1 | reservoir | 0.6 (M) | ||
| 6 | 1 | reservoir | EDTA | 1.0 (mM) |
