1EYN
Structure of mura liganded with the extrinsic fluorescence probe ANS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1999-11-01 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.100, 66.600, 107.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.330 - 1.700 |
Rwork | 0.180 |
R-free | 0.21000 |
Starting model (for MR) | 1ejc |
RMSD bond length | 0.017 |
RMSD bond angle | 24.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.330 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.062 | 0.290 |
Total number of observations | 1222364 * | |
Number of reflections | 49890 | |
<I/σ(I)> | 20 | |
Completeness [%] | 99.5 | 98.3 |
Redundancy | 24 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 | 292 | MES/PEG20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | Mes/NaOH | 25 (mM) | |
2 | 1 | 1 | PEG20000 | 5 (%) | |
3 | 1 | 1 | ANS | 2.5 (mM) |