1EYN
Structure of mura liganded with the extrinsic fluorescence probe ANS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-01 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.100, 66.600, 107.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.330 - 1.700 |
| Rwork | 0.180 |
| R-free | 0.21000 |
| Starting model (for MR) | 1ejc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 24.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.330 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.062 | 0.290 |
| Total number of observations | 1222364 * | |
| Number of reflections | 49890 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 99.5 | 98.3 |
| Redundancy | 24 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 292 | MES/PEG20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Mes/NaOH | 25 (mM) | |
| 2 | 1 | 1 | PEG20000 | 5 (%) | |
| 3 | 1 | 1 | ANS | 2.5 (mM) |
