1EYI
FRUCTOSE-1,6-BISPHOSPHATASE COMPLEX WITH MAGNESIUM, FRUCTOSE-6-PHOSPHATE AND PHOSPHATE (R-STATE)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 120 |
Detector technology | AREA DETECTOR |
Collection date | 1998-08-01 |
Detector | SIEMENS |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 52.120, 82.510, 165.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.320 |
Rwork | 0.190 |
R-free | 0.24200 |
Starting model (for MR) | 1cnq |
RMSD bond length | 0.005 |
RMSD bond angle | 22.600 * |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.460 |
High resolution limit [Å] | 2.320 | 2.320 |
Rmerge | 0.640 | 0.239 |
Total number of observations | 72680 * | |
Number of reflections | 30028 | |
<I/σ(I)> | 15.6 | |
Completeness [%] | 96.1 | 75.5 |
Redundancy | 4.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 37 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | 10 (mM) | ||
3 | 1 | drop | 5 (mM) | or 5 mM MgCl2 | |
4 | 1 | drop | F6P | (mM) | |
5 | 1 | reservoir | Tris-malonate | (mM) | |
6 | 1 | reservoir | PEG3350 | (%(w/v)) |