1EYG
Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 1998-03-25 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.688, 71.081, 79.160 |
| Unit cell angles | 90.00, 91.93, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.256 |
| Rwork | 0.256 |
| R-free | 0.29800 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.035 * | 0.080 * |
| Total number of observations | 44241 * | |
| Number of reflections | 13271 * | |
| Completeness [%] | 97.6 * | 96.7 * |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 291 | PEG 4000,PEG 200, Hepes, pH 7.5, vapor diffusion, macro seeding, temperature 291K, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Hepes | ||
| 2 | 1 | 1 | PEG 200 | ||
| 3 | 1 | 1 | PEG 4000 | ||
| 4 | 1 | 2 | PEG 200 | ||
| 5 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 100 (mM) | |
| 2 | 1 | reservoir | PEG200 | 30 (%(v/v)) | |
| 3 | 1 | reservoir | PEG4000 | 20 (%(v/v)) |
