1EYC

STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T41I/S59A/P117G/H124L/S128A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	297
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 41
Unit cell lengths	49.305, 49.305, 63.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.850
R-factor	0.1674 *
R-free	0.25030
Structure solution method	AB INITIO
RMSD bond length	0.006
RMSD bond angle	0.024
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	1.850 *
Completeness [%]	92.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	MPD, Sodium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	40-60 (%(v/v))
2	1	reservoir	sodium phosphate	25 (mM)