1EXR
THE 1.0 ANGSTROM CRYSTAL STRUCTURE OF CA+2 BOUND CALMODULIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-07-21 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.015, 29.415, 52.761 |
| Unit cell angles | 89.54, 86.10, 82.39 |
Refinement procedure
| Resolution | 50.000 * - 1.000 |
| R-factor | 0.1345 * |
| R-free | 0.16300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNPUBLISHED 1.1 A PARAMECIUM TETRAURELIA CALMODULIN |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.073 | 0.506 |
| Number of reflections | 78247 | 6757 * |
| <I/σ(I)> | 17.4 | 2.1 |
| Completeness [%] | 96.9 | 83.9 |
| Redundancy | 5.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 4 * | MPD, calcium chloride, sodium cacodylate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | CaM | 15 (mg/ml) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 4 | 1 | drop | MPD | 0.001ml | |
| 5 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 6 | 1 | reservoir | MPD | 50 (%) |
