1EXM
CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS ELONGATION FACTOR TU (EF-TU) IN COMPLEX WITH THE GTP ANALOGUE GPPNHP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-21 |
Temperature [K] | 298 |
Detector technology | DIFFRACTOMETER |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.500, 99.500, 40.200 |
Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
Resolution | 14.780 - 1.700 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.24300 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | MADNESS |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | X-PLOR |
Refinement software | CNS (1.0) |
Data quality characteristics
Outer shell | |
Low resolution limit [Å] | 1.810 |
High resolution limit [Å] | 1.700 |
Completeness [%] | 43.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20mM Sodium cacodylate, 5mM Magnesium sulfate, 38-44% Ammonium sulfate, 7-10 mg/mL EF-Tu, molar ratio EF-Tu:GppNHp = 1:5, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20mM Sodium cacodylate, 5mM Magnesium sulfate, 38-44% Ammonium sulfate, 7-10 mg/mL EF-Tu, molar ratio EF-Tu:GppNHp = 1:5, pH 7.0, VAPOR DIFFUSION, HANGING DROP |