1EXD
CRYSTAL STRUCTURE OF A TIGHT-BINDING GLUTAMINE TRNA BOUND TO GLUTAMINE AMINOACYL TRNA SYNTHETASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-01-17 |
Detector | OTHER |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 236.940, 93.980, 113.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.700 |
Rwork | 0.216 |
R-free | 0.28900 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | X-PLOR (98.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.070 | 0.407 |
Number of reflections | 34389 | |
<I/σ(I)> | 7.2 | |
Completeness [%] | 97.7 | 93.9 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | (NH4)2SO4, Hepes, b-mercaptoethanol, MgSO4 at 298 K, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | b-mercaptoethanol | ||
2 | 1 | 1 | Hepes | ||
3 | 1 | 1 | MgSO4 | ||
4 | 1 | 1 | (NH4)2SO4 | ||
5 | 1 | 2 | MgSO4 | ||
6 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | magnesium chloride | 5 (mM) | |
3 | 1 | drop | GlnRS | 4.5 (mg/ml) | |
4 | 1 | drop | PIPES | 7.5 (mM) | |
5 | 1 | drop | beta-mercaptoethanol | 2.5 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
7 | 1 | reservoir | PIPES | 70 (mM) | |
8 | 1 | reservoir | 20 (mM) |