1EXB
STRUCTURE OF THE CYTOPLASMIC BETA SUBUNIT-T1 ASSEMBLY OF VOLTAGE-DEPENDENT K CHANNELS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-22 |
Detector | BRANDEIS - B4 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 100.725, 100.725, 110.901 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.100 |
R-factor | 0.216 |
Rwork | 0.215 |
R-free | 0.22700 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.080 | 0.300 |
Number of reflections | 33500 | |
<I/σ(I)> | 17.8 | |
Completeness [%] | 98.8 | 92.9 |
Redundancy | 11.8 | 9689 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.6 | 277 | Peg 4000, glycine, pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | glycine | 50 (mM) | |
3 | 1 | reservoir | PEG400 | 6 (%) |