1EX6
CRYSTAL STRUCTURE OF UNLIGANDED FORM OF GUANYLATE KINASE FROM YEAST
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-03-24 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 31 |
Unit cell lengths | 51.559, 51.559, 119.645 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.164 * |
Rwork | 0.175 |
R-free | 0.25900 |
Structure solution method | HGMAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.017 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.080 | 0.543 |
Total number of observations | 37285 * | |
Number of reflections | 15759 | |
<I/σ(I)> | 9.9917 | 1.5845 |
Completeness [%] | 99.7 | 97.9 |
Redundancy | 2.37 | 2.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 100 * | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
4 | 1 | reservoir | sodium acetate | 0.3 (M) | |
5 | 1 | reservoir | Tris-HCl | 0.1 (M) |