1EX3
CRYSTAL STRUCTURE OF BOVINE CHYMOTRYPSINOGEN A (TETRAGONAL)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04-17 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 114.900, 114.900, 52.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.204 |
Rwork | 0.204 |
RMSD bond length | 0.021 |
RMSD bond angle | 2.900 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.092 | 0.268 |
Number of reflections | 7446 | |
<I/σ(I)> | 17.63 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.16 | 5.59 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | other * | 5 | 25 * | 0.1 M NaCl, 10 mM Na citrate, 0.05% Na azide, 1 mM phenylmethanesulphonyl-fluoride, pH 5.0, Centrifugal crystallization, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 10 (g/L) | |
2 | 1 | 1 | 0.1 (M) | ||
3 | 1 | 1 | sodium citrate | 10 (mM) | |
4 | 1 | 1 | sodium azide | 0.05 (%) | |
5 | 1 | 1 | PMSF | 1 (mM) |