1EX2

CRYSTAL STRUCTURE OF BACILLUS SUBTILIS MAF PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	1998-09-05
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	62.660, 86.010, 93.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.850
Rwork	0.195
R-free	0.22300
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.068	0.449
Total number of observations	186600 *
Number of reflections	43834
<I/σ(I)>	17
Completeness [%]	99.4	96.3
Redundancy	4.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir	PEG8000	8 (%)