1EX1
BETA-D-GLUCAN EXOHYDROLASE FROM BARLEY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE M18X |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 1997-05-15 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.090, 102.090, 184.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.17 * |
Rwork | 0.170 |
R-free | 0.20200 |
Structure solution method | MIRAS |
RMSD bond length | 0.013 |
RMSD bond angle | 0.035 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.054 | 0.142 |
Total number of observations | 148526 * | |
Number of reflections | 40310 * | |
<I/σ(I)> | 20.1 | 2.8 |
Completeness [%] | 78.0 | 32 * |
Redundancy | 3.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 277-279 * | Hrmova, M., (1998) Acta Cryst., D54, 687. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 6.8 (mg/ml) | |
2 | 1 | drop | HEPES-NaOH | 75 (mM) | |
3 | 1 | drop | sodium acetate | 7.5 (mM) | |
4 | 1 | drop | PEG400 | 1.2 (%(w/v)) | |
5 | 1 | drop | ammonium sulfate | 0.8 (M) | |
6 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
7 | 1 | reservoir | HEPES-NaOH | 50 (mM) |