1EX0
HUMAN FACTOR XIII, MUTANT W279F ZYMOGEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.540, 70.640, 133.380 |
| Unit cell angles | 90.00, 106.08, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.000 |
| Rwork | 0.187 |
| R-free | 0.23400 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (V 3.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.510 | 1.840 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.048 | 0.402 |
| Number of reflections | 158697 | |
| <I/σ(I)> | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 10 - 12 % 1,2-propanediol, 0.1 M Na-K phosphate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
