1EWY
ANABAENA PCC7119 FERREDOXIN:FERREDOXIN-NADP+-REDUCTASE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.730, 63.720, 158.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.380 |
| R-factor | 0.219 * |
| Rwork | 0.219 |
| R-free | 0.29200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QUE FOR FERREDOXIN-NADP+REDUCTASE |
| RMSD bond length | 0.022 * |
| RMSD bond angle | 0.022 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.380 | 2.280 |
| Rmerge | 0.046 | 0.077 |
| Total number of observations | 175226 * | |
| Number of reflections | 25838 | |
| <I/σ(I)> | 7.9 | 4.2 |
| Completeness [%] | 97.2 | 87.7 |
| Redundancy | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 293 * | 20-24% PEG 6000,10 mM MES pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oxidized FNR | 0.7 (mM) | |
| 2 | 1 | drop | oxidized Fd | 0.5 (mM) | |
| 3 | 1 | drop | Tris-HCl | 0.1 (M) | pH8.0 |
| 4 | 1 | drop | beta-octylglucoside | 5 (%) | |
| 5 | 1 | reservoir | MES | 0.1 (M) | pH5.5 |
| 6 | 1 | reservoir | PEG6000 | 18-21 (%(w/v)) | |
| 7 | 1 | reservoir | sodium azide | 0.1 (%(w/v)) |
