1EWH
STRUCTURE OF CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-27 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.450, 94.300, 119.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.25600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.080 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.059 | 0.035 |
| Total number of observations | 128030 * | |
| Number of reflections | 35084 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 95.2 | 91 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 * | 293 | PEG 8000, calcium acetate, MES, pH 6.5, VAPOR DIFFUSION, temperature 20K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 88 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | MES | 100 (mM) | |
| 5 | 1 | reservoir | calcium acetate | 25-50 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 12-18 (%(w/v)) |
