1EW2

CRYSTAL STRUCTURE OF A HUMAN PHOSPHATASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE D41A
Synchrotron site	LURE
Beamline	D41A
Temperature [K]	80
Detector technology	IMAGE PLATE
Collection date	1997-04-24
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	88.800, 114.500, 106.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.000 - 1.820
R-factor	0.185
Rwork	0.185
R-free	0.24200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.000	1.740
High resolution limit [Å]	1.820 *	1.820 *
Rmerge	0.082 *	0.488 *
Number of reflections	41585 *
<I/σ(I)>	8.5
Completeness [%]	79.6	67.6 *
Redundancy	2.34	1.32

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6	293	PEG, pH 6.0, VAPOR DIFFUSION, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-16 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop		2 (mM)
4	1	drop		0.02 (%)
5	1	reservoir	PEG4000	12 (%)
6	1	reservoir	zinc acetate	2 (mM)
7	1	reservoir	imidazole malate	200 (mM)