1EW0

CRYSTAL STRUCTURE ANALYSIS OF THE SENSOR DOMAIN OF RMFIXL(FERROUS FORM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06-10
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	60.300, 37.040, 53.450
Unit cell angles	90.00, 115.44, 90.00

Refinement procedure

Resolution	20.000 - 1.400
R-factor	0.205
Rwork	0.205
R-free	0.26500
RMSD bond length	0.009
RMSD bond angle	1.527
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.280
High resolution limit [Å]	1.200	1.200
Rmerge	0.051	0.278
Total number of observations	165005 *
Number of reflections	33489
<I/σ(I)>	28.7
Completeness [%]	92.4	84.4
Redundancy	4.93	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5 *	20 *	PEG 4000, acetic acid/NaOH, ammonium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mM)
2	1	reservoir	sodium acetate	100 (mM)
3	1	reservoir	ammonium acetate	200 (mM)
4	1	reservoir	PEG4000	40 (%(w/v))