1EVZ
CRYSTAL STRUCTURE OF LEISHMANIA MEXICANA GLYCEROL-3-PHOSPHATE DEHYDROGENASE IN COMPLEX WITH NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 125 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-20 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.850, 69.850, 211.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.25500 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.230 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.930 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.066 | 0.183 |
Total number of observations | 93667 * | |
Number of reflections | 13000 | |
<I/σ(I)> | 27.63 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 6.8 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 * | 298 | Triethanol amine pH 7.25 Sodium Citrate 0.9M, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3.5 (mg/ml) | |
2 | 1 | drop | TEA | 20 (mM) | |
3 | 1 | drop | NAD | 4 (mM) | |
4 | 1 | drop | dithiothreitol | 4 (mM) | |
5 | 1 | drop | EDTA | 1 (mM) | |
6 | 1 | drop | PMSF | 0.2 (mM) | |
7 | 1 | reservoir | sodium citrate | 0.9 (M) |