1EVT
CRYSTAL STRUCTURE OF FGF1 IN COMPLEX WITH THE EXTRACELLULAR LIGAND BINDING DOMAIN OF FGF RECEPTOR 1 (FGFR1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-25 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 |
Unit cell lengths | 62.553, 64.061, 64.139 |
Unit cell angles | 93.40, 111.17, 97.18 |
Refinement procedure
Resolution | 25.000 - 2.800 |
R-factor | 0.249 |
Rwork | 0.249 |
R-free | 0.30000 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.960 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.083 | 0.226 |
Total number of observations | 49288 * | |
Number of reflections | 22330 * | |
<I/σ(I)> | 8.6 | |
Completeness [%] | 97.9 | 90.5 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | PEG 4000, Isopropanol, HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 0.1 (M) | |
3 | 1 | reservoir | PEG4000 | 30 (%) | |
4 | 1 | reservoir | 0.2 (M) | ||
5 | 1 | reservoir | Tris-HCl | 0.1 (M) |