1EVR
The structure of the resorcinol/insulin R6 hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-03-19 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.350, 61.926, 47.805 |
| Unit cell angles | 90.00, 110.61, 90.00 |
Refinement procedure
| Resolution | 44.750 - 1.900 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.21800 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 20.900 * |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.750 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | |
| Total number of observations | 81864 * | |
| Number of reflections | 25793 | |
| <I/σ(I)> | 18.4 | |
| Completeness [%] | 96.9 | 93.1 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.7 | 298 | 30 mg insulin, 3.0 ml 0.02 M HCl, 0.3 ml 0.15 M Zinc Acetate, 1.5 ml 0.2 M Sodium Citrate, 1.2 ml 5% Resorcinol in water, 0.36 gm NaCl, pH 6.7, SLOW COOLING, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | insulin | 30mg | |
| 2 | 1 | 1 | 0.02 (M) | 3.0ml | |
| 3 | 1 | 1 | zinc acetate | 0.15 (M) | 0.3ml |
| 4 | 1 | 1 | sodium citrate | 0.2 (M) | 1.5ml |
| 5 | 1 | 1 | resorcinol in water | 5 (%) | |
| 6 | 1 | 1 | 0.36g |
