1EVK
CRYSTAL STRUCTURE OF A TRUNCATED FORM OF THREONYL-TRNA SYNTHETASE WITH THE LIGAND THREONINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.050, 117.340, 87.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.000 |
| R-factor | 0.2138 |
| Rwork | 0.212 |
| R-free | 0.24100 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.039 | 0.297 |
| Total number of observations | 282694 * | |
| Number of reflections | 73054 | |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 98.2 | 95.4 |
| Redundancy | 3.87 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 4000, ammonium acetate, magnesium chloride , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ThrRS | ||
| 2 | 1 | drop | tRNAThr | ||
| 3 | 1 | drop | ammonium acetate | ||
| 4 | 1 | drop | ATP | ||
| 5 | 1 | reservoir | PEG4000 | precipitant |
