1EVI
THREE-DIMENSIONAL STRUCTURE OF THE PURPLE INTERMEDIATE OF PORCINE KIDNEY D-AMINO ACID OXIDASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-04 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.730, 91.370, 71.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.239 |
| Rwork | 0.232 |
| R-free | 0.29800 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.210 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.067 | 0.151 |
| Total number of observations | 86285 * | |
| Number of reflections | 23734 | |
| <I/σ(I)> | 18 | |
| Completeness [%] | 96.5 | 95.9 |
| Redundancy | 3.56 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 20 * | Setoyama, C., (1996) J. Biochem., 1114. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 120 (mM) | |
| 3 | 1 | drop | sodium citrate | 60 (mM) | |
| 4 | 1 | drop | sodium benzoate | 0.2 (mM) | |
| 5 | 1 | drop | PEG4000 | 5 (%) | |
| 6 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 7 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 8 | 1 | reservoir | PEG4000 | 30 (%) |
