1EVH

EVH1 DOMAIN FROM MURINE ENABLED IN COMPLEX WITH ACTA PEPTIDE

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	200
Detector technology	CCD
Collection date	1998-11-15
Detector	ADSC
Wavelength(s)	0.96859, 0.99984
Spacegroup name	C 2 2 21
Unit cell lengths	33.614, 62.566, 94.316
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.229 *
Rwork	0.230
R-free	0.26800
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.090 *	0.358 *
Total number of observations	88290 *
Number of reflections	17747
<I/σ(I)>	11
Completeness [%]	99.1 *	91.4 *
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5	20 *	0.1 M NA HEPES, 70% SATURATED AMMONIUM PHOSPHATE, pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	SeMet Mrna 1-112	15 (mg/ml)
2	1	drop	AC-FPPPPT-CONH2	6 (mM)
3	1	reservoir	ammonium phosphate	60 (%sat)
4	1	reservoir	HEPES	0.1 (M)