1EVG
CRYSTAL STRUCTURE ANALYSIS OF CYS167 MUTANT OF ESCHERICHIA COLI WITH UNMODIFIED CATALYTIC CYSTEINE
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-25 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | I 21 3 |
| 格子定数 [Å] | 131.973, 131.973, 131.973 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 6.000 - 2.000 |
| R因子 | 0.212 |
| Rwork | 0.212 |
| R-free | 0.22500 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 24.000 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 6.000 | 2.070 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.083 | 0.214 |
| 独立反射数 | 24936 | |
| <I/σ(I)> | 25.79 | |
| 完全性 [%] | 100.0 | 99.6 |
| 冗長性 | 10.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 291 | 48% saturated ammonium sulfate, 100 mM Tris HCl, 20 mM 2-mercaptoethanol , pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | enzyme | 7-10 (mg/ml) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | 2-mercaptoethanol | 15 (mM) | |
| 4 | 1 | drop | potassium chloride | 50-100 (mM) | |
| 5 | 1 | drop | potassium phosphate | 20-50 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 48 (%sat) | |
| 7 | 1 | reservoir | 2-mercaptoethanol | 20 (mM) | |
| 8 | 1 | reservoir | Tris-HCl | 100 (mM) |
