1EV5
CRYSTAL STRUCTURE ANALYSIS OF ALA167 MUTANT OF ESCHERICHIA COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 138 |
Detector technology | CCD |
Collection date | 1998-11-25 |
Detector | OTHER |
Spacegroup name | I 21 3 |
Unit cell lengths | 132.491, 132.491, 132.491 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.700 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.21900 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 6.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.056 | 0.450 |
Total number of observations | 459585 * | |
Number of reflections | 41527 | |
<I/σ(I)> | 46.57 | |
Completeness [%] | 99.9 * | 0.62 |
Redundancy | 11.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 291 | 38% saturated ammonium sulfate, 100 mM Tris HCl, 40 mM sodium phosphate, 20 mM 2-mercaptoethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 7-10 (mg/ml) | |
2 | 1 | drop | EDTA | 1 (mM) | |
3 | 1 | drop | 2-mercaptoethanol | 15 (mM) | |
4 | 1 | drop | potassium chloride | 50-100 (mM) | |
5 | 1 | drop | potassium phosphate | 20-50 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 48 (%sat) | |
7 | 1 | reservoir | potassium phosphate | 100 (mM) |