1EV1
ECHOVIRUS 1
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 113 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 472.150, 483.200, 352.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.550 |
| R-factor | 0.263 |
| Rwork | 0.263 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 24.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.660 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Rmerge | 0.136 | 0.241 |
| Total number of observations | 3522138 * | |
| Number of reflections | 947283 | |
| Completeness [%] | 97.7 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 6 * | 277 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | virus | 10 (mg/ml) | |
| 10 | 1 | reservoir | PEG400 | 2.5 (%) | |
| 2 | 1 | 1 | sucrose | 30 (%) | |
| 3 | 1 | 1 | 1 (mM) | ||
| 4 | 1 | 1 | PIPES | 10 (mM) | |
| 5 | 1 | 1 | 25 (mM) | ||
| 6 | 1 | 1 | 1 (mM) | ||
| 7 | 1 | reservoir | PIPES | 10 (mM) | |
| 8 | 1 | reservoir | 25 (mM) | ||
| 9 | 1 | reservoir | 25 (mM) |
