1EUY
GLUTAMINYL-TRNA SYNTHETASE COMPLEXED WITH A TRNA MUTANT AND AN ACTIVE SITE INHIBITOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-04-12 |
Detector | OTHER |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 235.200, 94.200, 113.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
Rwork | 0.234 |
R-free | 0.27700 |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.590 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.056 | 0.274 |
Total number of observations | 309007 * | |
Number of reflections | 48345 | |
<I/σ(I)> | 0.151 | |
Completeness [%] | 95.5 | 95.2 |
Redundancy | 3 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 298 | 2.1 M (NH4)2SO4, 70 mM Buffer, 20 mM MgCl2, 20 mM BME at 298 K, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Buffer | ||
2 | 1 | 1 | BME | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | (NH4)2SO4 | ||
5 | 1 | 2 | MgCl2 | ||
6 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
10 | 1 | reservoir | beta-mercaptoethanol | 20 (mM) | |
2 | 1 | drop | PIPES | 10 (mM) | |
3 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
4 | 1 | drop | tRNA mutant | 2-5 (mg/ml) | |
5 | 1 | drop | 5 (mM) | ||
6 | 1 | drop | QSI | 2 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 2.1 (M) | |
8 | 1 | reservoir | PIPES | 70 (mM) | |
9 | 1 | reservoir | 20 (mM) |