1EUM

CRYSTAL STRUCTURE OF THE E.COLI FERRITIN ECFTNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-12-12
Detector	MARRESEARCH
Spacegroup name	I 4
Unit cell lengths	129.920, 129.920, 173.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.200 - 2.050
R-factor	0.188
Rwork	0.186
R-free	0.24700
RMSD bond length	0.007
RMSD bond angle	0.629
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.200	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.058	0.430
Total number of observations	202488 *
Number of reflections	87413
<I/σ(I)>	8.6
Completeness [%]	97.7	100
Redundancy	2.3	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	5.2	20 *	20 mM Piperazine HCl Buffer, 1mM EDTA, 0.1 - 0.6M NaCl, pH 5.2, MICRODIALYSIS, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	piperazine/HCl	20 (mM)
2	1	1	EDTA	1 (mM)
3	1	1		0.1-0.6 (M)