1EUE
RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-15 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.230, 70.770, 72.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 11.930 - 1.800 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1awp |
RMSD bond length | 0.010 |
RMSD bond angle | 21.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.200 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.065 | 0.480 |
Number of reflections | 21091 | |
<I/σ(I)> | 20.6 | 1.8 |
Completeness [%] | 93.0 | 91.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.8 | 277 | drop solution is mixed 1:1 by volume with precipitant solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 20 (%(w/v)) | precipitant |
3 | 1 | reservoir | magnesium acetate | 0.2 (M) | precipitant |
4 | 1 | reservoir | PIPES | 0.1 (M) | precipitant |