1EUC

CRYSTAL STRUCTURE OF DEPHOSPHORYLATED PIG HEART, GTP-SPECIFIC SUCCINYL-COA SYNTHETASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	DIFFRACTOMETER
Collection date	1993-12-01
Detector	WEISSENBERG
Spacegroup name	P 1 21 1
Unit cell lengths	86.798, 81.973, 49.891
Unit cell angles	90.00, 104.53, 90.00

Refinement procedure

Resolution	59.000 - 2.100
R-factor	0.172
Rwork	0.158
R-free	0.22000
RMSD bond length	0.018
RMSD bond angle	1.900
Data reduction software	WEIS
Data scaling software	WEIS
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.000	2.130
High resolution limit [Å]	2.100	2.090
Rmerge	0.109	0.307
Total number of observations	213996 *
Number of reflections	37771
Completeness [%]	95.4	71
Redundancy	4	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS		4 *	sodium,potassium(1:3) phosphate, 2-mercaptoethanol, ammonium sulfate, MICRODIALYSIS, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	11-14 (mg/ml)
2	1	2	ammonium sulfate	1.65-1.68 (M)
3	1	2	2-mercaptoethanol	1 (mM)
4	1	2	Na/K(1:3)phosphate	43 (mM)