1EU5
STRUCTURE OF E. COLI DUTPASE AT 1.45 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-28 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 85.821, 85.821, 60.476 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.000 - 1.450 |
| R-factor | 0.1401 |
| Rwork | 0.183 |
| R-free | 0.23220 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.032 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.043 | 0.150 |
| Number of reflections | 29458 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.2 * | 277 | SUCCINATE PH 5.5, BIS-TRIS, PH 6.2, PEG 8000, EVAPORATION, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | succinate | 5 (mM) | |
| 2 | 1 | 1 | sodium ethylmercurythiosalicylate | 100 (mM) | |
| 3 | 1 | 2 | protein | 3 (mg/ml) | |
| 4 | 1 | 2 | Bis-Tris | 10 (mM) | |
| 5 | 1 | 2 | PEG8000 | 1.5 (%) |
