1EU1
THE CRYSTAL STRUCTURE OF RHODOBACTER SPHAEROIDES DIMETHYLSULFOXIDE REDUCTASE REVEALS TWO DISTINCT MOLYBDENUM COORDINATION ENVIRONMENTS.
Replaces: 1CXSReplaces: 1CXTExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 1999-02-04 |
| Detector | ADSC |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.176, 141.718, 59.812 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.300 |
| R-factor | 0.121 * |
| Rwork | 0.121 |
| R-free | 0.14500 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 0.030 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.051 | 0.211 |
| Number of reflections | 191461 | |
| <I/σ(I)> | 19.9 | |
| Completeness [%] | 89.8 | 60.2 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 295 | Hepes, Cadmium Chloride, Ammonium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 22.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 10 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.65 (M) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | |
| 5 | 1 | reservoir | 10 (mM) |
