1ETW
THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT G72D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-12 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.330, 50.260, 47.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.230 - 2.000 |
| Rwork | 0.231 |
| R-free | 0.27900 |
| Starting model (for MR) | The wild-type FIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 18.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 * | 0.301 * |
| Total number of observations | 76866 * | |
| Number of reflections | 12915 | |
| <I/σ(I)> | 31.8 | |
| Completeness [%] | 99.2 | 98.9 |
| Redundancy | 5.95 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 9 mg/ml protein, 0.5 M sodium chloride, 0.1M magnesium acetate, 0.05M sodium cacodylate(pH 6.5), 10% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 1 (M) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | reservoir | magnesium acetate | 0.1 (M) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 6 | 1 | reservoir | PEG8000 | 20 (%) |
